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Project Title:
Transfusion Medicine Hemostaisis Clinical Research Network
Funding Agency:
National Institutes of Health
Total Project Period:
Sep 30, 2002 - Aug 31, 2007
Principal Investigator:
Joseph Carcillo, MD
Project Summary:
In year 6 (2003-04), we propose to explore in dogs in study I slower exsanguination over 30 min. to near-CA, and to maximize preservation time for transport and repair. For study I-A we hypothesize that survival time after near-CA followed by hypotensive FR is longer under 34°C by external cooling than under normothermia, and longer with the novel poikilothermia inducing peptide neurotensin, as compared to morphine. In study I-B we hypothesize that following exsanguination to near-CA, a period of prolonger hypotensive FR at 34°C is less preservative than the same period of CA at 10°C by aortic cold flush of a special solution. In study I-C we propose to maximize preservation time after exsanguination to near-CA over 30 min, by combining in sequence hypotensive FR for uncontrolled HS of 30 min vs 60 min vs 90 min followed by SA for CA of 90 min at 10°C.
Study II in rats on the limits of resuscitability will use brain mitochondrial proteomics. We will identify indicators of cell death during CA and reperfusion at various temperatures.
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