Titles:
Assistant Professor, CCM

Contact:
Email: sappingtonpl@ccm.upmc.edu

Degrees:

Major Honors/Awards:

• Expert Faculty Appreciation (Mid-Atlantic AIDS Education DC Local Performance Site, Howard University); 1997-1998.
• First Place Poster Presentation for “Mortality in Patients with Gram Negative Sepsis versus Gram Positive Sepsis in Disseminated Intravascular Coagulation” (Howard University Scientific Research Forum);1999.
• Second Place Poster Presentation for “Gram Negative Bacteremias in HIV-Infected Patients” (Howard University Scientific Research Forum); 1999.
• Society of Critical Care Medicine: Internal Medicine Specialty Award for Abstract: “Ethyl Pyruvate Ameliorates HMGB1 B Box(B)-induced Intestinal Epithelial Barrier Dysfunction;” 2004.

Research Grants:

Current Research Interests:

• Ethyl Pyruvate
• HMGB1 Alteration Tight Junction Structure and Function
• Center for Cardiac Stem Cell Therapy
• VAP in Lung and Lung-Heart Transplant Patients
• Nitric Oxide Synthase Inhibitor in Cardiac Patients

Publications:

• Khan A, Delude R, Han Y, Sappington P, Carcillo J, Fink M.  Liposomal NAD+ prevents diminished O2 uptake by immunostimulated Caco-2  cells.  Am J Physiol Lung Cell Mol Physiol  2002; 282(5)::L1082-L1091.
• Sappington P, Yang R, Yang H, Tracey K, Delude R, Fink M.  HMG-1 B-Box increases the permeability of Caco-2 enterocytic monolayers and impairs intestinal barrier function in mice.  Gastroenterology  2002; 123(3)::790-802.
• Sappington P, Han X, Yang R, Delude R, Fink M.  Ethyl pyruvate ameliorates intestinal epithelial barrier dysfunction in endotoxemic mice and imunostimulated Caco-2 enteroctyic monolayers. J Pharm Exp Therap  2003; 304(1)::464-476.
• Berg S, Sappington P, Delude R, Fink M.  Pro-inflammatory cytokines increase the rate of glycolysis and ATP turnover in cultured rat enterocytes. Crit Care Med  2003; 31(4)::1203-1212.
• Han X, Uchiyama T, Sappington P, Yaguchi A, Yang R, Fink M, Delude R.  NAD+ ameliorates inflammation-induced epithelial barrier dysfunction in cultured enterocytes and mouse ileal mucosa.  J Pharm Exp Therap  2003; 307(2)::443-449.
• Sappington PL, Fink ME, Yang R, Delude RL, Fink MP.  Ethyl pyruvate provides durable protection against inflammation-induced intestinal epithelial barrier dysfunction. Shock 2003; 20(6)::521-528.
• Sappington PL, Cruz RJ, Harada T, Yang R, Han Y, Englert JA, Ajami AA, Killeen ME, Delude RL, Fink MP.  The ethyl pyruvate analogues, diethyl oxaloproprionate, 2-acetamidoacrylate, and methyl-2-acetamidoacrylate, exhibit anti-inflammatory properties in vivo and/or in vitro.  Biochem Pharm  2005; 70(11)::1579-1592.
• Liu S, Stolz DB, Sappington PL, Macias CA, Killeen ME, Tenhunen JJ, Delude RL, Fink MP.  HMGB1 is secreted by immunostimulated enterocytes and contributes to cytomix-induced hyperpermeability of Caco-2 monolayers. Am J Physiol Cell Physiol 2006; 290(4):C990-9.
• Raman KG, Sappington PL, Yang R, Levy RM, Prince JM, Liu S, Watkins SK, Schmidt AM, Billiar TR, Fink MP.  The role of RAGE in the pathogenesis of intestinal barrier dysfunction after hemorrhagic shock.  Am J Physiol Gastrointest Liver Physiol  2006; 291:556-565.

Presentations at Major Meetings:

• “Recombinant HMG-1 “B-Box” Increases the Permeability of CACO-2 Enterocytic Monolayers Via a RAGE-Dependent Mechanism,” Shock Meeting, Marco Island, FL  (1997)
•  “Mortality in Patients with Gram Negative Sepsis versus Gram Positive Sepsis in Disseminated Intravascular Coagulation.,” Howard University Scientific Research Forum, Washington, DC  (1999)
•  “Schistosomiasis in a Large Inner City Teaching Hospital,” Annual Meeting of the American Tropical Disease Medicine of Hygiene, Washington, DC  (1999)
•  “HMGB1 B Box Increases the Permeability of Cac0-2 Enterocytic Monolayers and Impairs Intestinal Barrier Function in Mice,” Eighth Annual Life and Physical Sciences Research Symposium. Greensboro, NC  (February 2003)
•  “HMGB1: A New Target in the Treatment of Sepsis,” Howard University Medical Grand Rounds, Washington, DC  (May 2003)

11-Apr-2007 dmk